英语翻译In an attempt to identify the function of specific class

英语翻译
In an attempt to identify the function of specific classIII peroxidases,several authors reported the generation of transgenic plants to study different peroxidase genes.However,in A.thaliana only 9 out of 73 peroxidase genes have been identified by this approach (Table 1).The characterisation of individual peroxidase mutants often gives only unconclusive results [8-12].The in planta role of most peroxidases remains therefore elusive.This situation is mainly linked to two difficulties inherent in peroxidases:i) gene redundancy results in no visible mutant phenotype,and ii) the lack of substrate specificity in vitro,prohibits a determination of which compounds are real in planta substrate.Molecular biology approaches seems to offer a powerful tool to overcome these difficulties [7].Transcriptome analysis for example provides a detailed description of the gene regulation during plant growth and development,in any plant tissue and also in different relevant genotypes.Thus a transcriptomic approach should permit an efficient screen showing which peroxidases are expressed at key developmental stages,but also to identify redundant peroxidases genes putatively involved in the same specific process in all kind of tissues,development stages and genotypes.Nevertheless,not all peroxidase genes are represented on commercially available microarrays.For this reason,in this study we used a home made macroarray dedicated exclusively to the 73 class III peroxidase genes of A.thaliana [13]

在试图确定具体classIII过氧化物酶的功能,一些作者报告说,转基因植物研究不同代过氧化物酶的基因.然而,在只有9拟南芥过氧化物酶的基因中,有73个已经确定了这一方针（见表1）.过氧化物酶突变体的个体特征往往只提供unconclusive结果[8-12].在大多数过氧化物酶作用的遗体足底因此elusive.This形势,主要是与两个固有的困难,过氧化物：在我没有明显的突变体）的基因冗余的结果,及ii）在体外特异性底物缺乏,其中一个决心禁止在植物基化合物是真实的.分子生物学的方法似乎提供了一个强大的工具来克服这些困难[7].转录例如分析提供了植物生长和发育过程中的任何植物组织,基因调控详细说明,而且在不同的基因型有关.因此,一个转录的方法应该允许一个有效的屏幕显示哪些过氧化物酶均以在关键的发展阶段,也是鉴定公认多余过氧化物酶在同一具体过程涉及在所有的组织,发展阶段和基因型的一种基因.然而,并非所有的代表过氧化物酶基因在商用芯片.出于这个原因,在此研究中,我们使用了自制的,专门的73个第三类过氧化物酶基因的拟南芥[13]宏阵列