作业帮英文文献翻译（英译汉）
来源：学生作业帮 编辑：作业帮 分类：英语作业 时间：2024/05/17 03:42:57
英文文献翻译（英译汉）
哪位英语高手可以帮我翻译一下这篇生物学方面的英文文献?不要直接用软件翻译,要准确通顺的翻译,谢谢.
Secondary structure in RNA targets is a significant barrier to short DNA probes. However, when such targets are the end product of an in vitro amplification scheme, it is possible to carry out transcription in the presence of nucleoside triphosphate analogues that reduce secondary structure of the RNA without impairing subsequent hybridization. Here we show that nucleoside triphosphates of 2-aminoadenine (nA) and 2-thiouracil (sU) are taken up by T7 RNA polymerase and that the resulting RNA possesses reduced secondary structure and improved accessibility to DNA probes. The hybridization properties of short RNA transcripts were studied using a new gel mobility shift assay from which melting temperatures were determined. RNA hairpins that contained nA and sU were able to hybridize to DNA probes under conditions where the unmodified hairpins did not. DNA-RNA hybrids that contained nA and sU in the RNA strand exhibited enhanced specificity, increased stability, and greater equality of base pairing strength than the same hybrids without modifications. Substitution of guanine (G) with inosine (I) further reduced secondary structure, but RNA with this base hybridized nonselectively. The high stability of nA-T and A-sU base pairs in DNA-RNA hybrids, combined with the destabilizing effect of the nA-sU couple in RNA targets, accounts for the improved hybridization properties. These results suggest that incorporation of nA and sU during in vitro transcription is a promising strategy for enhancing the performance of oligomeric DNA probes with an RNA target.
哪位英语高手可以帮我翻译一下这篇生物学方面的英文文献?不要直接用软件翻译,要准确通顺的翻译,谢谢.
Secondary structure in RNA targets is a significant barrier to short DNA probes. However, when such targets are the end product of an in vitro amplification scheme, it is possible to carry out transcription in the presence of nucleoside triphosphate analogues that reduce secondary structure of the RNA without impairing subsequent hybridization. Here we show that nucleoside triphosphates of 2-aminoadenine (nA) and 2-thiouracil (sU) are taken up by T7 RNA polymerase and that the resulting RNA possesses reduced secondary structure and improved accessibility to DNA probes. The hybridization properties of short RNA transcripts were studied using a new gel mobility shift assay from which melting temperatures were determined. RNA hairpins that contained nA and sU were able to hybridize to DNA probes under conditions where the unmodified hairpins did not. DNA-RNA hybrids that contained nA and sU in the RNA strand exhibited enhanced specificity, increased stability, and greater equality of base pairing strength than the same hybrids without modifications. Substitution of guanine (G) with inosine (I) further reduced secondary structure, but RNA with this base hybridized nonselectively. The high stability of nA-T and A-sU base pairs in DNA-RNA hybrids, combined with the destabilizing effect of the nA-sU couple in RNA targets, accounts for the improved hybridization properties. These results suggest that incorporation of nA and sU during in vitro transcription is a promising strategy for enhancing the performance of oligomeric DNA probes with an RNA target.
Secondary structure in RNA targets is a significant barrier to short DNA probes.However,when such targets are the end product of an in vitro amplification scheme,it is possible to carry out transcription in the presence of nucleoside triphosphate analogues that reduce secondary structure of the RNA without impairing subsequent hybridization.
目标RNA的二级结构对短DNA探针来说是一个严重的障碍,可是,如果这些靶RNA是人工体外增殖的产物,并且在三磷酸核苷存在的环境下,改变RNA的二级结构,DNA可以进行转录,并且不会影响杂交产物的特性
非专业人士瞎翻的,下班了,没空了.
目标RNA的二级结构对短DNA探针来说是一个严重的障碍,可是,如果这些靶RNA是人工体外增殖的产物,并且在三磷酸核苷存在的环境下,改变RNA的二级结构,DNA可以进行转录,并且不会影响杂交产物的特性
非专业人士瞎翻的,下班了,没空了.