在GENBANK上面查到的线粒体DNA序列,接下来怎么设计PCR引物?

在GENBANK上面查到的线粒体DNA序列,接下来怎么设计PCR引物?
序列如下,是CYTB的
>gi|207266417:13707-14849 Falco tinnunculus mitochondrion, complete genome
ATGGCACCCAACATTCGAAAATCACACCCCCTAATAAAAATAATCAACAACTCCCTAATCGACCTCCCCA
CCCCATCCAACATCTCCATATGATGAAACTTTGGATCCCTACTAGGAATCTGCCTAGCCACCCAAATCTT
AACCGGCCTACTATTAGCCATACACTACACAGCAGACACAACTCTAGCCTTCTCATCCGTCGCCCACACA
TGTCGAAACGTACAGTACGGATGATTAATCCGCAACCTACACGCCAACGGAGCATCACTATTCTTTATTT
GCATTTACATGCACATCGGACGGGGCATCTACTACGGCTCCTATCTGTACAAAGAAACATGAAACACAGG
CATCATCCTTCTACTCACCCTAATAGCAACAGCCTTCGTCGGCTATGCATTACCCTGAGGACAGATATCA
TTCTGAGGCGCCACAGTAATCACCAACTTATTCTCAGCAATCCCATACATTGGCCAAACCCTAGTTGAAT
GGGCCTGAGGAGGGTTCTCAGTAGACAACCCAACGCTAACCCGATTCTTCGCCCTACACTTTCTCCTACC
ATTCATTATTGCAGGACTCACCCTAATCCACCTCACCTTCCTACACGAATCAGGCTCAAACAACCCCCTA
GGAGTAGCATCAAACTGCGATAAAATCCCATTCCACCCCTACTACTCCCTAAAAGACCTCCTAGGATTCA
TACTCATATACCTCCCCCTAATAGCCCTAGCCCTATTCACCCCAAACCTACTAGGCGACCCAGAAAACTT
TACACCAGCAAACCCCCTAGTCACCCCCCCACACATCAAACCAGAATGGTATGTCCTATTTGCCTACGCC
ATCCTACGCTCAATTCCCAACAAACTAGGATGAGTCCTAGCACTAGCTGCCTCAGTATTAATCTTATTCC
TAAACCCACTACTCCACAAATCTAAACAGCGCACAATAACCTTCCGCCCATTATCTCAATCACTATTCTG
ACTCCTAATCACTAACCTACTTATCCTAACCTGAATTGGAAGCCAACCCGTCGAACAACCATTCATCATT
ATTGGACAACTAGCCTCCCTCTCCTACTTCACCATCCTCCTAATCCTCCTCCCCCTTACCGGAGCCCTAG
AAAACAAAATCCTCAACTACTAA
我想PCR线粒体细胞色素B的这段,但是不会用PRIMER之类的软件,请问是否只要取前后各20个左右的碱基然后AT,GC这样子反过来就是引物了?
还有这段碱基序列到底是什么东西的啊?是线粒体DNA还是mRNA的?
谢谢了
网址如下
http://www.ncbi.nlm.nih.gov/nuccore/NC_011307.1?report=fasta&from=13707&to=14849
http://www.ncbi.nlm.nih.gov/gene/6947145#

当然是dna的啦,引物的设计有很多需要注意的地方哦,不然怎么有primer5 、oligo7之类设计引物软件呢.