谁帮我把这段文章翻译成英文,翻译好的多给分!

谁帮我把这段文章翻译成英文,翻译好的多给分!
采用Sanger双脱氧链末端终止法,按T7 Sequencing TM Kit使用说明操作.[n一 P]
dATP标记.8% 变性聚丙烯酰胺凝胶,厚度0．4mm,胶长55cm,电泳在Pharmach LKB公司的Macrophor测序仪上进行,1200V~1500V,2～5小时.测序产物置x
光负片爆光,于一20℃过夜,放射自显影.
AT PJtts基固克隆与表达 将pPGAP —AT用NcoI—SalI双酶切,插入pBV220中,得到pBV220一a1一AT重组质粒.再将其中的a1-AT(BamHl—Ava1)片段用PCR定点诱变后的突变片段(BamHI—AvaI)置换,即得到垒基目的pBV220 AT P[tts表达质粒.该
质粒转化大肠杆菌HB101,在LB培养基中30"C生长至对数中期．42"C诱导3小时.收集菌体,超声破碎后分别用1mol／L,2mol／L尿素洗涤,8mol/L尿素溶解
包涵体.

比较靠谱的翻译：
采用Sanger双脱氧链末端终止法,按T7 Sequencing TM Kit使用说明操作.[n一 P] dATP标记.8% 变性聚丙烯酰胺凝胶,厚度0．4mm,胶长55cm,电泳在Pharmach LKB公司的Macrophor测序仪上进行,1200V~1500V,5小时.测序产物置x光负片爆光,于一20℃过夜,放射自显影.
Sanger’s dideoxynucleotide chain terminating method was adopted and conducted according to the application instructions of T7 Sequencing TM Kit.Marked as [n一 P] dATP.The electrophoresis of 8%denaturing polyacrylamide gel with 0.4mm thickness and 55cm in length was conducted on Pharmach LKB Company’s Macrophor DNA sequencing machine at 1200V~1500V for 5 hours.The sequenced product was then subject to X-ray negative film explosion light and autoradiography for overnight at - 20°C.
AT PJtts基固克隆与表达:将pPGAP —AT用NcoI—SalI双酶切,插入pBV220中,得到pBV220一a1一AT重组质粒.再将其中的a1-AT(BamHl—Ava1)片段用PCR定点诱变后的突变片段(BamHI—AvaI)置换,即得到垒基目的pBV220 AT P[tts表达质粒.
AT PJtts gene cloning and expression:NcoI—SalI double enzyme digestion was conducted on pPGAP —AT,and it was inserted into pBV220 vector to obtain pBV220一a1一AT recombinant plasmid.One of the a1-AT(BamHl—Ava1) gene fragments was displaced by (BamHI—Ava1) which was a mutated fragment from PCR site directed mutagenesis process,and thus barrier based purpose pBV220 AT P[tts expression plasmids were obtained.
该质粒转化大肠杆菌HB101,在LB培养基中30"C生长至对数中期．42"C诱导3小时.收集菌体,超声破碎后分别用1mol／L,2mol／L尿素洗涤,8mol/L尿素溶解
包涵体.
The plasmids were transformed into Escherichia coli HB101,they were cultured in LB medium at 30"C until mid-logarithmic growth and induced for 3 hours at 42"C.The thalli were collected and subject to ultrasonication; they were separately cleansed by using 1mol／L and 2mol／L urea,and then dissolved in 8 mol/L urea.
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