英语翻译nThe extraction of RNA was performed using the RNeasy mi

来源：学生作业帮编辑：作业帮分类：英语作业时间：2024/05/27 18:53:32

英语翻译
nThe extraction of RNA was performed using the RNeasy mini kit n(Qiagen).RT-qPCRs for RNA expression were performed by using nthe KAPA SYBR FAST One-step RT-qPCR kit (KAPA biosystems).nRT-qPCRs were performed on anMx3005p Real Time PCR System n(Stratagene).All PCR products were verified on agarose gels nstained with ethidium bromide.We designed and synthesized nthe following primers:ACE1-sense,5′-caacatcaccacagagaccagcaa- n3′; ACE1-antisense,5′-ggtgtctcgtacatagacctccac-3′; reninsense,n5′-caatccgagaaagcctgaag-3′; renin-antisense,5′-ctgtgtcaccgtgattccac- n3′; GAPDH-sense,5′-cgagatccctccaaaatcaa-3′; and nGAPDH-antisense,5′-gtcttctgggtggcagtgat-3′

RNA的提取是用Qiagen公司生产的RNeasy 迷你试剂盒.

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