英语翻译医学方向..,翻译器有道神马的就别来了~了RNA interference.Although procedure

英语翻译
医学方向..,翻译器有道神马的就别来了~了
RNA interference.Although procedureshave been previously described37,38,
RNAi experiments with adult parasites were based on methods optimized for
schistosomula39.Briefly,in vitro cultured parasites were soaked with 20–30 mg
of dsRNA freshly added on days 1–3 and every 5–6days thereafter.As a negative
control,animals were soaked with dsRNA synthesized from the ccdB and camRcontaining
insert of pJC53.2 (ref.29).dsRNA synthesis was performed as previously
described29.Sequences used to generate dsRNAs are provided in
Supplementary Fig.9.To measure mRNA levels,tot

试译如下,供参考：
RNA interference.
RNA（核糖核酸）干扰.
Although procedures have been previously described [37,38],RNAi experiments with adult parasites were based on methods optimized for schistosomula [39].
尽管先前已对程序作了描述[37,38],成人寄生虫RNAi（核糖核酸干扰）实验是基于血吸虫童虫的优化方法的[39].
Briefly,in vitro cultured parasites were soaked with 20–30 mg of dsRNA freshly added on days 1–3 and every 5–6 days thereafter.
简言之,将体外培育的寄生虫在第1–3天用新添加的20–30毫克双链RNA浸泡,随后每5–6天添加.
As a negative control,animals were soaked with dsRNA synthesized from the ccdB and camR containing insert of pJC53.2 (ref.29).
用从含有pjc53.2添加物的ccdB和camR合成得到的双链RNA对动物进行浸泡以进行阴性对照（参考29）.
dsRNA synthesis was performed as previously described [29].Sequences used to generate dsRNAs are provided in Supplementary Fig.9.
双链RNA的合成是按之前描述的那样进行的[29].用于产生双链RNA的顺序见补充图9.
To measure mRNA levels,total RNA from control and knockdown parasites (,8 male posterior somatic fragments) was reverse transcribed (iScript cDNA Synthesis kit,Bio-Rad) and quantitative real-time PCR was performed on an Applied Bio-systems Step One Plus instrument using GoTaq qPCR Master Mix with SYBR green (Promega).
为测量的mRNA（信使核糖核酸）水平,将对照和解体寄生虫中的总RNA（8个阳性后体碎片）进行反转录（BioRad合成试剂盒）,并用含SYBR绿色试剂盒的GoTaq定量多聚酶链反应(qPCR)超级混合包在应用生物系统一步加的仪器上进行实时定量聚合酶链反应(PCR).
Transcript levels were normalized to the mRNA levels of proteasome subunit beta type-4 (smp_056500).
将转录水平按4型蛋白酶体β亚基的mRNA水平（smp_056500）进行规范校正.
Relative quantities were calculated using the DDCt calculation in the Step One Plus software.Oligonucleotide primer sequences are listed in Supplementary Table 3.
用一步加(Step One Plus)软件中的 DDCt计算法对相对量进行计算.寡核苷酸基本序列列于补充表3中.