英语翻译Purification of Neutral Sphingomyelinase 2 from Bovine B

英语翻译
Purification of Neutral Sphingomyelinase 2 from Bovine Brain and Its Calcium-dependent Activation.
Kim SK,Ahn KH,Jeon HJ,Lee DH,Jung SY,Jung KM,Kim DK.
Department of Environmental & Health Chemistry,College of Pharmacy,Chung-Ang University,221 Huksuk-dong,Dongjak-ku,Seoul 156-756,South Korea.
Ceramide is produced by sphingomyelinase (SMase) and it plays a key role in cellular responses such as apoptosis.In this study,we report the purification and characterization of neutral SMase2 (nSMase2) from bovine brain tissue.Triton X-100 extracts of bovine brain membranes were purified in nine steps,including sequential chromatography.The specific activity of purified nSMase increased 8183-fold over the brain membrane fraction.Purified nSMase showed similarities to nSMase2,which had been purified and cloned previously.Interestingly,purified nSMase2 was Ca(2+)-dependent and could be activated by micromolar concentrations of Ca(2+) under Mg(2+)-free conditions.Ceramide generation was dependent upon the calcium ionophore A23187 and was observed in nSMase2-overexpressing COS-7 cells.This generation was suppressed by GW4869,an nSMase2 inhibitor,but not to FB1,an inhibitor of the de novo ceramide synthesis pathway.The present study demonstrates the Ca(2+)-dependent activation of nSMase2.
PMID:19968763 [PubMed - as supplied by publisher]

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中性鞘2纯化牛脑及钙依赖激活.
金水库,安哈诺夫,全黄建忠,利生,郑马西荣公里,金不知道.
清华大学环境及健康化学,药剂学,书院,中央大学,221 Huksuk洞,铜雀区,首尔156-756,韩国.
神经酰胺是由鞘磷脂（磷脂酶）,并播放,如细胞凋亡的细胞反应的一个关键作用.在这项研究中,我们报告的净化和中立SMase2（nSMase2从牛脑组织）特性.仓鼠- 100的牛脑提取纯化膜在9个步骤,其中包括顺序色谱.
纯化nSMase的具体活动增加了8183倍,脑细胞膜的一小部分.纯化nSMase显示相似之处nSMase2,已被纯化和克隆以前.有趣的是,纯化nSMase2是钙（2 +）依赖,可以由微摩尔浓度的钙激活（2 +）根据镁（2 +）无条件.神经酰胺一代depe
ndent呼吁钙离子载体A23187,并在nSMase2观察高表达COS - 7细胞.这一代是抑制GW4869,一nSMase2抑制剂,而不是FB1,一个神经酰胺抑制剂从头合成途径.本研究表明钙（2 +）依赖性激活nSMase2.
日期：19968763 [医学-由出版商提供]