英语翻译Nanoparticle Preparation.Conjugated polymer was dissolve

英语翻译
Nanoparticle Preparation.Conjugated polymer was dissolved
by stirring overnight in THF under inert atmosphere.The solution
was then filtered through a 1.6 ím filter and further diluted to a
concentration of 20 ppm.A 2 mL quantity of the polymer/THF
solution was added quickly to 8 mL of deionized water while
sonicating the mixture.The suspension was filtered with a 0.2 ím
membrane filter.The THF was removed by partial evaporation under
vacuum,followed by filtration through a 0.2 ím filter.The overall
yield of the nanoparticles was typically higher than 80%.The resulting
nanoparticle dispersions were clear,with colors similar to those of
the polymers in THF solution.
Silica Encapsulation.The silica encapsulation is achieved by a
two-step method.At the first step of nanoparticle preparation,0.05
g of OTMOS in ethanol solution (0.08%) was added to 10 mL of
a 5 ppm nanoparticle dispersion before the evaporation of THF,then
rocked for 30 min.THF was then removed by partial vacuum
evaporation.Further growth of a silica shell is based on the
polymerization of active silica.Asolution of active silica was prepared
by lowering the pH of a 0.54% sodium silicate solution to 10-11
by the progressive addition of cation-exchange resin (Amberlite
IR120,16-50 mesh).29,30 A 0.5 mL portion of active silica was
added to a 10 mL suspension of silane-modified polymer nanoparticles.
The mixture was allowed to stand for 24 h under vigorous
stirring so that the active silica polymerized onto the nanoparticle
surface.The silica shell thickness was about 2-4 nmafter 24 h.The
dispersion was then dialyzed against deionized water for 48 h to
remove excess silica.
Formation of Gold Nanoparticle Assemblies.Gold nanoparticles
were prepared according to the standard sodium citrate reduction
method.31 A 1.0 mL portion of the sodium citrate solution (34 mM)
was added to 50 mL of boiled chlorauric acid solution (0.25 mM)
while stirring.The obtained gold nanoparticles had an average
diameter of 15 nm with 10% size dispersity.A silane coupling agent
(APTMS) was used to assemble the silica-coated polymer nanoparticles
with gold nanoparticles.A 20 íL portion of the APTMS
solution (0.2 wt %) was added to a suspension of silica-encapsulated
PFPV nanoparticles (5 mL,16 nM) and rocked for 1 h.The amount
of APTMS was calculated to coat the nanoparticles with one
monolayer.A 5 mL portion of the amine-functionalized PFPV
nanoparticles (16nM)was then mixed with 5mLof gold nanoparticles
(1 nM).The mixture was allowed to stand for 2 h prior to spectroscopic
measurements.A similar procedure was used to assemble MEHPPV
with gold nanoparticles,and the resulting dispersion contained
MEH-PPV and gold with 12 nM and 0.5 nM concentrations,
respectively

纳米粒子的制备.共轭聚合物在惰性气体氛围下,于THF（四氢呋喃）中搅拌一个晚上加以溶解.然后将该溶液通过一个1.6im的过滤器过滤,并进一步稀释到20ppm的浓度.将2mL数量的聚合物/THF溶液迅速加到8mL的去离子水中,同时声波降解（sonicating）该混合物.该悬浮液用一个0.2im的膜过滤器过滤.THF用部分蒸发在真空下去除,然后哦通过一个0.2im的过滤器过滤.纳米粒子的整个产率典型来说能超过80％.最终的纳米粒子分散体是清澈的,颜色类似于聚合物在THF溶液中的颜色.
二氧化硅包封.二氧化硅包封是通过两步法实现的.在纳米粒子制备的第一步,在乙醇溶液中的0.05gOTMOS（正辛基三乙氧基硅烷）（0.08％）被加到10mL的5ppm纳米粒子分散体内,然后让THF蒸发,再摇晃30min.然后将THF用部分真空蒸发方法去除.二氧化硅
壳层的进一步生长是基于活性二氧化硅的聚合作用.活性二氧化硅的溶液是将0.54％的硅酸钠（水玻璃）溶液的pH值通过逐步添加阳离子交换树脂（Amberlite IR120,16－50孔目）降低到10－11而制备的.一份0.5mL的活性二氧化硅被添加到10mL的硅烷改性聚合物纳米粒子的悬浮液中.让该混合物放置在激烈的搅拌下24h,从而使活性二氧化硅聚合到纳米粒子表面上.二氧化硅壳层的厚度在24h后大约是2～4nm.然后将悬浮液对去离子水透析48h,以去除过多的二氧化硅.
金纳米粒子组件的形成.金纳米粒子是根据标准的柠檬酸钠还原法制备的.将一份1.0mL的柠檬酸钠溶液（34mM）加入50mL的沸腾氯金酸溶液（0.25mM）中,同时搅拌.所得到的金纳米粒子具有15nm的平均直径,尺寸分散性为10％.硅烷偶联剂（APTMS）被用于将二氧化硅涂覆的聚合物纳米粒子与金纳米粒子组合起来.一份20iL的APTMS溶液（0.2％质量分数）被二氧化硅包封的PFPV纳米粒子（5mL,16nM）的悬浮液中,并摇晃1h.APTMS的量加以计算以涂覆具有一层单分子层的纳米粒子.然后,一份5mL的胺官能化PFPV纳米粒子（16nM）用5mL的金纳米粒子（1nM）混合.该混合物放置2h,然后进行分光测量.类似的步骤也被用于组合含有金纳米粒子的MEH－PPV,得到的悬浮液分别含有12nM浓度的MEH-PPV和0.5nM浓度的金.