英语翻译2.5.Fungicidal bioassayThe tested fungi including Cortic

英语翻译
2.5.Fungicidal bioassay
The tested fungi including Corticum rolfsii f.sp.(Curzi,
Roma N.S.),Fusarium solani (MAFF 237472) and Fusarium
oxysporum (Mormodiace,S.K.Sun & J.W.Huang)
were kindly provided from the Laboratory of Plant Pathology,
University of the Ryukyus,Okinawa,Japan.C.rolfsii,
F.solani and F.oxysporum were isolated from Mangifera
indica,Nicotiana tabacum L.and Balsam pear plants,
respectively.The three phytopathogens have been characterized
by causing the following symptoms:basal rot disease
of tomato,brown root rot in peanuts and fusarium
yellows on asparagus.These phytopathogens were cultured
on potato dextrose agar (PDA) (1.5% agar) for 4 days at
27 \3C before the experiment was carried out.One ml volume
of each diluted solution was mixed with 8.0 ml of
PDA and was then put in the Petri dish.Finally,the spores
from a mycelia disk (5.7 mm in diameter) were put on the
centre of the PDA plate.All plates were put into an incubator
(27 \3C) for 4 days and controls contained 10% Tween
80 only.The antifungal activity was measured by agar dilution
method (Taira,Tawata,Kobamoto,Toyama,& Yasuda,
1994) and expressed as percentage inhibition against
the mycelia growth diameter.

2.5 .杀菌生物活性
测试的真菌包括corticum rolfsii f.sp.（ curzi ,
罗姆的NS ） ,茄根腐病菌（ maff 237472 ）和镰刀菌
枯萎病（ mormodiace ,圣公会孙＆黄振文）
被好心提供了从实验室的植物病理学,
大学的琉球,日本冲绳.长rolfsii ,
楼菌和F病菌中分离出芒果
籼,烟草研究和苦瓜植物,
分别.三phytopathogens已特点
造成以下症状：基腐病
番茄,褐根腐病在花生和镰刀菌
黄对芦笋.这些phytopathogens培养
对马铃薯葡萄糖琼脂（ PDA ）的（ 1.5 ％琼脂）为四天在
27实验前进行了研究.1毫升货量
每个稀释溶液混合8.0毫升的
PDA和当时提出,在培养皿中.最后,孢子
从菌丝体磁盘（ 5.7 mm的直径）提上了
中心的PDA的钢板.所有车牌被放进一个孵化器
（ 27 c ）为四天和控制载10 ％ ,吐温
80只.该抗真菌活性测定琼脂稀释法
方法（平,tawata ,kobamoto ,富山,＆安田,
1994年） ,并表示作为百分比抑制对
该菌丝生长的直径.