医学文献翻译求助Genomic DNA for Figs 1, 3 and 4 were collected at 2–

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医学文献翻译求助Genomic DNA for Figs 1, 3 and 4 were collected at 2–4 days post-fertilization from
Genomic DNA for Figs 1, 3 and 4 were collected at 2–4 days
post-fertilization from 24–32 individual larvae by incubating in 50mM NaOH at
95 uC, followed by cooling to 4 uC and adding 1/10 volume 1M Tris-HCL pH8.0
(ref. 25). Genomic DNA for Fig. 2 was isolated from groups of 10 larval zebrafish
using DNeasy Blood and Tissue kit (Qiagen).

你在看什么文献呢.
图1,3.4的基因组DNA是从受精2-4天后的24-32个不同的幼体里提取的,通过在50mM NaOH里95℃孵育,然后冷却至4℃,加入1/10体积的1M Tris-HCL pH8.0（参考文献25）.
图2的基因组DNA是使用DNeasy Blood and Tissue kit (Qiagen,一种DNA提取试剂盒)从不同组别的10个幼体斑马鱼中分离得到的.

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