您还在做BrdU实验方法吗
来源:学生作业帮 编辑:作业帮 分类:英语作业 时间:2024/05/11 01:21:28
您还在做BrdU实验方法吗
你说的是细胞周期的流式细胞伩检测实验方法(Brdu)?
操作步骤:
1. Cells (1x106/mL) are incubated with BrdU 10 mM at final concentration, for 30 min at 37 °C in controlled atmosphere.
2.Wash twice at 500 g for 1 min using the washing buffer.
3. Resuspend in 0.5 mL of washing buffer and 0.5 mL of HCl 4 M.
4. Mix accurately and incubate for 30 min at room temperature.
5. Wash once as in step 2.
6. Resuspend in 1 mL of Borax buffer.
7. As in step 5.
8. Resuspend in 200 mL of washing buffer and label with 5 mL of mAb ant-BrdU.
9. Incubate for 1 hour at 4 °C in the dark.
10. As in step 5.
11. Resuspend in 200 mL of washing buffer and label with 4 mL of goat-anti-mouse FITC-conjugated antibody.
12. Incubate for 30 min at 4 °C in the dark.
13. As in step 5.
14. Resuspend in 200 mL of washing buffer and 200 mL of PI buffer.
15. Incubate for 15-30 min at 4 °C in the dark.
16. Analyse with flow cytometer equipped with a 488 nm argon laser.
操作步骤:
1. Cells (1x106/mL) are incubated with BrdU 10 mM at final concentration, for 30 min at 37 °C in controlled atmosphere.
2.Wash twice at 500 g for 1 min using the washing buffer.
3. Resuspend in 0.5 mL of washing buffer and 0.5 mL of HCl 4 M.
4. Mix accurately and incubate for 30 min at room temperature.
5. Wash once as in step 2.
6. Resuspend in 1 mL of Borax buffer.
7. As in step 5.
8. Resuspend in 200 mL of washing buffer and label with 5 mL of mAb ant-BrdU.
9. Incubate for 1 hour at 4 °C in the dark.
10. As in step 5.
11. Resuspend in 200 mL of washing buffer and label with 4 mL of goat-anti-mouse FITC-conjugated antibody.
12. Incubate for 30 min at 4 °C in the dark.
13. As in step 5.
14. Resuspend in 200 mL of washing buffer and 200 mL of PI buffer.
15. Incubate for 15-30 min at 4 °C in the dark.
16. Analyse with flow cytometer equipped with a 488 nm argon laser.
BrdU检测细胞增殖的方法
原料药中非降解杂质,在制剂方法学实验中还需要验证吗
从事化学分析的准妈妈,还有继续做实验的吗?我现在还在做简单的实验!有很大的影响吗?
BrdU能代替胸腺嘧啶脱氧核苷掺入到新合成的DNA链中.若用姬姆萨染料染色,在染色单体中,DNA只有一条单链掺有BrdU
BrdU能代替胸腺嘧啶脱氧核苷掺入到新合成的DNA链中.若用姬姆萨染料染色,在染色单体中,DNA只有一条单链掺有BrdU
如果在做凸透镜成像实验时,凸透镜被摔掉一块,还能在光屏上看到蜡烛完整的象吗?
在做还原糖鉴定实验中,选择甘蔗为实验材料,实验过程和方法规范,但没有得到理想的实验结果,原因是?
模拟实验是科学研究常用的一种方法,它是在难以_______做实验时,有时用________来做实验,即模仿_______
牛顿第一定律用的是什么实验方法,还有什么用这种方法做的实验?限在八上以前
在做什么真空是否传声的实验中,用到的试验方法是
在做探究求合力的方法的实验时,只用一个
王飞在做实验时不小心把凸透镜摔掉一部分,还能用它继续作实验吗?它还具有凸透镜的性质和特点吗?(只写出你的猜想即可)