作业帮求高手翻译一下这段英文
来源：学生作业帮 编辑：作业帮 分类：综合作业 时间：2024/05/17 01:33:56
求高手翻译一下这段英文
Partial purification of a-amylase from culture supernatant of Bacillus subtilis in aqueous two-phase systems
A study was made of the partition and purification of α-amylase from a culture supernatant of Bacillus subtilis in the polyethylene glycol (PEG)—citrate aqueous two-phase system (ATPS). Factors that influenced the partition of the protein in this system, including the molecular weight of the PEG, the tie line length of ATPS, the pH value and the sodium chloride concentration, were investigated. Purification of α-amylase was attained with a purification factor (PF) of 1.8 and 90% yield at pH 6.0 in a PEG1000-citrate ATPS with short tie line length. By utilizing the salt-out effect of neutral salt, the purification of α-amylase was further improved to 2.0 of PF and 80% yield in a PEG3350-citrate ATPS with 4% sodium chloride.
Partial purification of a-amylase from culture supernatant of Bacillus subtilis in aqueous two-phase systems
A study was made of the partition and purification of α-amylase from a culture supernatant of Bacillus subtilis in the polyethylene glycol (PEG)—citrate aqueous two-phase system (ATPS). Factors that influenced the partition of the protein in this system, including the molecular weight of the PEG, the tie line length of ATPS, the pH value and the sodium chloride concentration, were investigated. Purification of α-amylase was attained with a purification factor (PF) of 1.8 and 90% yield at pH 6.0 in a PEG1000-citrate ATPS with short tie line length. By utilizing the salt-out effect of neutral salt, the purification of α-amylase was further improved to 2.0 of PF and 80% yield in a PEG3350-citrate ATPS with 4% sodium chloride.
部分纯化从枯草芽孢杆菌的培养液培养,在双水相体系淀粉酶
一项研究是由分区和α-淀粉酶的分离纯化,在聚乙二醇（PEG）-柠檬酸枯草芽孢杆菌培养液双水相体系（双水相）.因素影响了在这个系统中的蛋白质,包括聚乙二醇,双水相并列的分子量分割线的长度,pH值和氯化钠浓度,
进行了调查.α-淀粉酶的分离纯化是达到了净化因子（PF）的1.8和90％的产率在pH短联络线长度1 PEG1000 -柠檬酸盐双水相6.0.由于使用的中性盐的盐效应,对α-淀粉酶的纯化得到进一步改善,酚醛2.0和80 4％氯化钠1 PEG3350 -柠檬酸盐双水相％的产率.
一项研究是由分区和α-淀粉酶的分离纯化,在聚乙二醇（PEG）-柠檬酸枯草芽孢杆菌培养液双水相体系（双水相）.因素影响了在这个系统中的蛋白质,包括聚乙二醇,双水相并列的分子量分割线的长度,pH值和氯化钠浓度,
进行了调查.α-淀粉酶的分离纯化是达到了净化因子（PF）的1.8和90％的产率在pH短联络线长度1 PEG1000 -柠檬酸盐双水相6.0.由于使用的中性盐的盐效应,对α-淀粉酶的纯化得到进一步改善,酚醛2.0和80 4％氯化钠1 PEG3350 -柠檬酸盐双水相％的产率.