作业帮英语翻译All isolates with MICs of ciprofloxacin of at least 4 μg
来源：学生作业帮 编辑：作业帮 分类：英语作业 时间：2024/05/08 00:07:04
英语翻译
All isolates with MICs of ciprofloxacin of at least 4 μg per milliliter were serotyped by the National Reference Center for Streptococcus (Edmonton,Alta.) with the use of type-specific antiserum.19 The genomic DNA of two groups of 12 and 14 randomly chosen isolates with MICs of ciprofloxacin of 1 μg per milliliter and 2 μg per milliliter,respectively,as well as all isolates with MICs of ciprofloxacin of at least 4 μg per milliliter,were examined by pulsed-field gel electrophoresis after digestion with SmaI in order to clarify clonal relationships.20,21 These isolates were further characterized by polymerase-chain-reaction and restriction-fragment–length polymorphism analysis with HinfI for the most commonly recognized mutations in parC and gyrA associated with resistance to fluoroquinolones that have previously been described in Staphylococcus aureus and Escherichia coli.22,23
All isolates with MICs of ciprofloxacin of at least 4 μg per milliliter were serotyped by the National Reference Center for Streptococcus (Edmonton,Alta.) with the use of type-specific antiserum.19 The genomic DNA of two groups of 12 and 14 randomly chosen isolates with MICs of ciprofloxacin of 1 μg per milliliter and 2 μg per milliliter,respectively,as well as all isolates with MICs of ciprofloxacin of at least 4 μg per milliliter,were examined by pulsed-field gel electrophoresis after digestion with SmaI in order to clarify clonal relationships.20,21 These isolates were further characterized by polymerase-chain-reaction and restriction-fragment–length polymorphism analysis with HinfI for the most commonly recognized mutations in parC and gyrA associated with resistance to fluoroquinolones that have previously been described in Staphylococcus aureus and Escherichia coli.22,23
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所有达到4微克/毫升环丙氟哌酸最小抑菌浓度（MIC）的分离菌株都由国家链球菌参考中心（埃德蒙顿,艾伯塔省）通过特定类型抗血清进行了血清分型（按表面抗原分类）.
两组分别为12个和14个随机选择的分离菌株——其MIC分别为1微克/毫升和2微克/毫升环丙氟哌酸,以及所有4微克/毫升环丙氟哌酸MIC的分离株,都在Smal内切酶消化处理后通过脉冲场凝胶电泳法（PFGE）测定了其染色体DNA,以便阐明其间的纯系关系.
通过多聚酶链式反应以及Hinfl（一种限制酶）限制性片段长度多态性分析,这些菌株被进一步按照通常存在于金黄色葡萄球菌及大肠杆菌中的parC和gyrA基因片段抗氟喹诺酮类药物变异进行了区分.
所有达到4微克/毫升环丙氟哌酸最小抑菌浓度（MIC）的分离菌株都由国家链球菌参考中心（埃德蒙顿,艾伯塔省）通过特定类型抗血清进行了血清分型（按表面抗原分类）.
两组分别为12个和14个随机选择的分离菌株——其MIC分别为1微克/毫升和2微克/毫升环丙氟哌酸,以及所有4微克/毫升环丙氟哌酸MIC的分离株,都在Smal内切酶消化处理后通过脉冲场凝胶电泳法（PFGE）测定了其染色体DNA,以便阐明其间的纯系关系.
通过多聚酶链式反应以及Hinfl（一种限制酶）限制性片段长度多态性分析,这些菌株被进一步按照通常存在于金黄色葡萄球菌及大肠杆菌中的parC和gyrA基因片段抗氟喹诺酮类药物变异进行了区分.
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